LOCUS       40924_5179        8137 bp DNA     circular SYN 17-DEC-2018
DEFINITION  PiggyBac transformation vector pB-MCS w+, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8137)
  AUTHORS   Griswold CM, Roebuck J, Andersen RO, Stam LF, Spana EP.
  TITLE     A toolkit for transformation and mutagenesis in Drosophila using 
            piggyBac
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8137)
  AUTHORS   Griswold CM, Roebuck J, Andersen RO, Stam LF, Spana EP.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2002) Invertebrate Targets, Syngenta 
            Biotechnology, Inc., 3054 Cornwallis Rd, Research Triangle Park, NC 
            27709, USA
REFERENCE   3  (bases 1 to 8137)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8137)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (13-DEC-2002) Invertebrate Targets, Syngenta Biotechnology, Inc., 
            3054 Cornwallis Rd, Research Triangle Park, NC 27709, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8137
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     mobile_element  complement(11..625)
                     /mobile_element_type="transposon:piggyBac 
                     transposableelement"
                     /label=ent
     primer_bind     642..658
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(692..708)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     gene            711..4830
                     /label=mini-white
                     /note="This modified version of the white gene lacks part
                     of the first intron."
     mobile_element  complement(4836..5284)
                     /mobile_element_type="transposon:piggyBac 
                     transposableelement"
                     /label=ent
     promoter        complement(5297..5315)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5336..5352)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(5360..5376)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(5384..5414)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(5429..5450)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(5738..6326)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(6500..7357)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(7358..7462)
                     /label=AmpR promoter
     rep_origin      complement(7488..7943)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     8085..8101
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        8111..8129
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gagctcttaa ccctagaaag ataatcatat tgtgacgtac gttaaagata atcatgcgta
       61 aaattgacgc atgtgtttta tcggtctgta tatcgaggtt tatttattaa tttgaataga
      121 tattaagttt tattatattt acacttacat actaataata aattcaacaa actatttatt
      181 tatgtttatt tatttattaa aaaaaaacaa aaactcaaaa tttcttctat aaagtaacaa
      241 aacttttaaa cattctctct tttacaaaaa taaacttatt ttgtacttta aaaacagtca
      301 tgttgtatta taaaataagt aattagctta acttatacat aatagaaaca aattatactt
      361 attagtcagt cagaaacaac tttggcacat atcaatatta tgctctcgac aaataacttt
      421 tttgcatttt ttgcacgatg catttgcctt tcgccttatt ttagaggggc agtaagtaca
      481 gtaagtacgt tttttcgtta ctggctcttc agtactgtca tctgatgtac caggcacttc
      541 atttggcaaa atattagaga tattatcgcg caaatatctc ttcaaagtag gagcttctaa
      601 acgcttacgc ataaacgatg acgtcccgcg gtggcggccg ctctagaact agtggatccc
      661 ccgggctgca ggaattcgat atcaagctta tcgataccgt cgacctcgag ctagtatgta
      721 tgtaagttaa taaaacccat ttttgcggaa agtagataaa aaaaacattt ttttttttta
      781 ctgcactgga tatcattgaa cttatctgat cagttttaaa tttacttcga tccaagggta
      841 tttgatgtac caggttcttt cgattacctc tcactcaaaa tgacattcca ctcaaagtca
      901 gcgctgtttg cctccttctc tgtccacaga aatatcgccg tctctttcgc cgctgcgtcc
      961 gctatctctt tcgccaccgt ttgtagcgtt acgtagcgtc aatgtccgcc ttcagttgca
     1021 ttttgtcagc ggtttcgtga cgaagctcca agcggtttac gccatcaatt aaacacaaag
     1081 tgctgtgcca aaactcctct cgcttcttat ttttgtttgt tttttgagtg attggggtgg
     1141 tgattggttt tgggtgggta agcaggggaa agtgtgaaaa atcccggcaa tgggccaaga
     1201 ggatcaggag ctattaattc gcggaggcag caaacaccca tctgccgagc atctgaacaa
     1261 tgtgagtagt acatgtgcat acatcttaag ttcacttgat ctataggaac tgcgattgca
     1321 acatcaaatt gtctgcggcg tgagaactgc gacccacaaa aatcccaaac cgcaattgca
     1381 caaacaaata gtgacacgaa acagattatt ctggtagctg ttctcgctat ataagacaat
     1441 ttttgagatc atatcatgat caagacatct aaaggcattc attttcgact atattctttt
     1501 ttacaaaaaa tataacaacc agatatttta agctgatcct agatgcacaa aaaataaata
     1561 aaagtataaa cctacttcgt aggatacttc ggggtacttt ttgttcgggg ttagatgagc
     1621 ataacgcttg tagttgatat ttgagatccc ctatcattgc agggtgacag cggagcggct
     1681 tcgcagagct gcattaacca gggcttcggg caggccaaaa actacggcac gctccggcca
     1741 cccagtccgc cggaggactc cggttcaggg agcggccaac tagccgagaa cctcacctat
     1801 gcctggcaca atatggacat ctttggggcg gtcaatcagc cgggctccgg atggcggcag
     1861 ctggtcaacc ggacacgcgg actattctgc aacgagcgac acataccggc gcccaggaaa
     1921 catttgctca agaacggtga gtttctattc gcagtcggct gatctgtgtg aaatcttaat
     1981 aaagggtcca attaccaatt tgaaactcag tttgcggcgt ggcctatccg ggcgaacttt
     2041 tggccgtgat gggcagttcc ggtgccggaa agacgaccct gctgaatgcc cttgcctttc
     2101 gatcgccgca gggcatccaa gtatcgccat ccgggatgcg actgctcaat ggccaacctg
     2161 tggacgccaa ggagatgcag gccaggtgcg cctatgtcca gcaggatgac ctctttatcg
     2221 gctccctaac ggccagggaa cacctgattt tccaagccat ggtgcggatg ccacgacatc
     2281 tgacctatcg gcagcgagtg gcccgcgtgg atcaggtgat ccaggagctt tcgctcagca
     2341 aatgtcagca cacgatcatc ggtgtgcccg gcagggtgaa aggtctgtcc ggcggagaaa
     2401 ggaagcgtct ggcattcgcc tccgaggctc taaccgatcc gccgcttctg atctgcgatg
     2461 agcccacctc cggactggac tcctttaccg cccacagcgt cgtccaggtg ctgaagaagc
     2521 tgtcgcagaa gggcaagacc gtcatcctga ccattcatca gccgtcttcc gagctgtttg
     2581 agctctttga caagatcctt ctgatggccg agggcagggt agctttcttg ggcactccca
     2641 gcgaagccgt cgacttcttt tcctagtgag ttcgatgtgt ttattaaggg tatctagtat
     2701 tacataacat ctcaactcct atccagcgtg ggtgcccagt gtcctaccaa ctacaatccg
     2761 gcggactttt acgtacaggt gttggccgtt gtgcccggac gggagatcga gtcccgtgat
     2821 cggatcgcca agatatgcga caattttgcc attagcaaag tagcccggga tatggagcag
     2881 ttgttggcca ccaaaaatct ggagaagcca ctggagcagc cggagaatgg gtacacctac
     2941 aaggccacct ggttcatgca gttccgggcg gtcctgtggc gatcctggct gtcggtgctc
     3001 aaggaaccac tcctcgtaaa agtgcgactt attcagacaa cggtgagtgg ttccagtgga
     3061 aacaaatgat ataacgctta caattcttgg aaacaaattc gctagatttt agatagaatt
     3121 gcctgattcc acacccttct tagttttttt caatgagatg tatagtttat agttttgcag
     3181 aagataaata aatttcattt aactcgcgaa tattaatgag atgcgagtaa cattttaatt
     3241 tgcagatggt tgccatcttg attggcctca tctttttggg ccaacaactc acgcaagtgg
     3301 gtgtgatgaa tatcaacgga gccatcttcc tcttcctgac caacatgacc tttcaaaacg
     3361 tctttgccac gataaatgta agtcatgttt agaatacatt tgcatttcaa taatttacta
     3421 actttctaat gaatcgattc gatttaggtg ttcacctcag agctgccagt ttttatgagg
     3481 gaggcccgaa gtcgacttta tcgctgtgac acatactttc tgggcaaaac gattgccgaa
     3541 ttgccgcttt ttctcacagt gccactggtc ttcacggcga ttgcctatcc gatgatcgga
     3601 ctgcgggccg gagtgctgca cttcttcaac tgcctggcgc tggtcactct ggtggccaat
     3661 gtgtcaacgt ccttcggata tctaatatcc tgcgccagct cctcgacctc gatggcgctg
     3721 tctgtgggtc cgccggttat cataccattc ctgctctttg gcggcttctt cttgaactcg
     3781 ggctcggtgc cagtatacct caaatggttg tcgtacctct catggttccg ttacgccaac
     3841 gagggtctgc tgattaacca atgggcggac gtggagccgg gcgaaattag ctgcacatcg
     3901 tcgaacacca cgtgccccag ttcgggcaag gtcatcccgg agacgcttaa cttctccgcc
     3961 gccgatctgc cgctggacta cgtgggtctg gccattctca tcgtgagctt ccgggtgctc
     4021 gcatatctgg ctctaagact tcgggcccga cgcaaggagt agccgacata tatccgaaat
     4081 aactgcttgt tttttttttt taccattatt accatcgtgt ttactgttta ttgccccctc
     4141 aaaaagctaa tgtaattata tttgtgccaa taaaaacaag atatgaccta tagaatacaa
     4201 gtatttcccc ttcgaacatc cccacaagta gactttggat ttgtcttcta accaaaagac
     4261 ttacacacct gcatacctta catcaaaaac tcgtttatcg ctacataaaa caccgggata
     4321 tattttttat atacatactt ttcaaatcgc gcgccctctt cataattcac ctccaccaca
     4381 ccacgtttcg tagttgctct ttcgctgtct cccacccgct ctccgcaaca cattcacctt
     4441 ttgttcgacg accttggagc gactgtcgtt agttccgcgc gattcggttc gctcaaatgg
     4501 ttccgagtgg ttcatttcgt ctcaatagaa attagtaata aatatttgta tgtacaattt
     4561 atttgctcca atatatttgt atatatttcc ctcacagcta tatttattct aatttaatat
     4621 tatgactttt taaggtaatt ttttgtgacc tgttcggagt gattagcgtt acaatttgaa
     4681 ctgaaagtga catccagtgt ttgttccttg tgtagatgca tctcaaaaaa atggtgggca
     4741 taatagtgtt gtttatatat atcaaaaata acaactataa taataagaat acatttaatt
     4801 tagaaaatgc ttggatttca ctggaactag acgtcatctt cacttacgtg atctgatatt
     4861 tcactgtcag aatcctcacc aacaagctcg tcatcgcttt gcagaagagc agagaggata
     4921 tgctcatcgt ctaaagaact acccatttta ttatatatta gtcacgatat ctataacaag
     4981 aaaatatata tataataagt tatcacgtaa gtagaacatg aaataacaat ataattatcg
     5041 tatgagttaa atcttaaaag tcacgtaaaa gataatcatg cgtcattttg actcacgcgg
     5101 tcgttatagt tcaaaatcag tgacacttac cgcattgaca agcacgcctc acgggagctc
     5161 caagcggcga ctgagatgtc ctaaacgcac agcgacggat tcgcgctatt tagaaagaga
     5221 gagcaatatt tcaagaatgc atgcgtcaat tttacgcaga ctatctttct agggttaagg
     5281 tacccagctt ttgttccctt tagtgagggt taattgcgcg cttggcgtaa tcatggtcat
     5341 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa
     5401 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc
     5461 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc
     5521 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact
     5581 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
     5641 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     5701 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     5761 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     5821 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     5881 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     5941 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     6001 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     6061 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     6121 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga
     6181 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     6241 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     6301 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     6361 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     6421 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     6481 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     6541 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     6601 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
     6661 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
     6721 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
     6781 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
     6841 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
     6901 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
     6961 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
     7021 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
     7081 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
     7141 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
     7201 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
     7261 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
     7321 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
     7381 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
     7441 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctaaa ttgtaagcgt
     7501 taatattttg ttaaaattcg cgttaaattt ttgttaaatc agctcatttt ttaaccaata
     7561 ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag accgagatag ggttgagtgt
     7621 tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg
     7681 aaaaaccgtc tatcagggcg atggcccact acgtgaacca tcaccctaat caagtttttt
     7741 ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa gggagccccc gatttagagc
     7801 ttgacgggga aagccggcga acgtggcgag aaaggaaggg aagaaagcga aaggagcggg
     7861 cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta accaccacac ccgccgcgct
     7921 taatgcgccg ctacagggcg cgtcccattc gccattcagg ctgcgcaact gttgggaagg
     7981 gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag
     8041 gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag
     8101 tgagcgcgcg taatacgact cactataggg cgaattg
//
